pMSP1D1T1
(Plasmid #97327)

• Purpose: Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
• Depositing Lab: Michael Marty
• Publication: Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192. doi: 10.1021/acs.analchem.7b03569. Epub 2017 Oct 23.

Backbone

• Vector backbone: pET 28a
• Backbone size w/o insert (bp): 5300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MSP1D1T1
• Alt name: D1T1
• Species: Synthetic
• Insert Size (bp): 639
• Mutation: Addition of T residue after T25
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis - TEV (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nco I (not destroyed)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: T7 Pro
• 3′ sequencing primer: T7 Term

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Stephen Sligar, https://www.addgene.org/20061/

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry