Engineering Nanodisc Scaffold Proteins for Native Mass Spectrometry.
Reid DJ, Keener JE, Wheeler AP, Zambrano DE, Diesing JM, Reinhardt-Szyba M, Makarov A, Marty MT
Anal Chem. 2017 Nov 7;89(21):11189-11192. doi: 10.1021/acs.analchem.7b03569. Epub 2017 Oct 23. PubMed Article
Reid DJ, Keener JE, Wheeler AP, Zambrano DE, Diesing JM, Reinhardt-Szyba M, Makarov A, Marty MT
Anal Chem. 2017 Nov 7;89(21):11189-11192. doi: 10.1021/acs.analchem.7b03569. Epub 2017 Oct 23. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 97323 | pMSP1D1R1 | Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry. |
| 97325 | pMSP1D1G1 | Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry. |
| 97327 | pMSP1D1T1 | Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry. |
| 97328 | pMSP1D1T2 | Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry. |