Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Danio alpha A-crystallin 1kb promoter/AcGFP
(Plasmid #98096)


Item Catalog # Description Quantity Price (USD)
Plasmid 98096 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4100
  • Total vector size (bp) 5137
  • Modifications to backbone
  • Vector type
    GFP expressing

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Alpha A-crystallin promoter 1 kb fragment, Danio rerio
  • Alt name
    Danio Cryaa
  • Alt name
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    cryaa (a.k.a. wu:fc06h04, zgc:92036)
  • Promoter Danio alpha A-crystallin 1 kb fragment (-1028/-1)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer TTCTTTCCTGCGTTATCC
  • 3′ sequencing primer CCATTCACATCGCCATTCA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Danio alpha A-crystallin 1kb promoter/AcGFP was a gift from Mason Posner (Addgene plasmid # 98096 ; ; RRID:Addgene_98096)
  • For your References section:

    The zebrafish as a model system for analyzing mammalian and native alpha-crystallin promoter function. Posner M, Murray KL, McDonald MS, Eighinger H, Andrew B, Drossman A, Haley Z, Nussbaum J, David LL, Lampi KJ. PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717/peerj.4093. eCollection 2017. 10.7717/peerj.4093 PubMed 29201567