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Mouse aB crystallin 250bp promoter/AcGFP
(Plasmid #98102)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98102 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 98102-D50 50 μg of DNA in Tris buffer $495
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DNA 98102-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pAcGFP1-1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4100
  • Total vector size (bp) 4374
  • Modifications to backbone
    None
  • Vector type
    GFP expressing

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Alpha B-crystallin promoter 250 bp fragment, Mus musculus
  • Alt name
    Mus Cryab
  • Alt name
    Cryab
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    259
  • GenBank ID
    CT010341
  • Entrez Gene
    Cryab (a.k.a. Crya-2, Crya2, HspB5, P23)
  • Promoter Alpha B-crystallin promoter 250 bp fragment (-259/-1)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer TTCTTTCCTGCGTTATCC
  • 3′ sequencing primer CCATTCACATCGCCATTCA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse aB crystallin 250bp promoter/AcGFP was a gift from Mason Posner (Addgene plasmid # 98102 ; http://n2t.net/addgene:98102 ; RRID:Addgene_98102)

  • For your References section:

    The zebrafish as a model system for analyzing mammalian and native alpha-crystallin promoter function. Posner M, Murray KL, McDonald MS, Eighinger H, Andrew B, Drossman A, Haley Z, Nussbaum J, David LL, Lampi KJ. PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717/peerj.4093. eCollection 2017. 10.7717/peerj.4093 PubMed 29201567
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