Mouse aB crystallin 800bp promoter/AcGFP
(Plasmid
#98103)
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PurposeMouse aB crystallin 800bp promoter segment driving GFP expression
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Depositing Lab
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Depositing OrganizationAshland University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 98103 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 98103-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 98103-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepAcGFP1-1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4100
- Total vector size (bp) 4942
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Modifications to backboneNone
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Vector typeGFP expressing
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAlpha B-crystallin promoter 800 bp fragment, Mus musculus
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Alt nameMus Cryab
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Alt nameCryab
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SpeciesM. musculus (mouse)
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Insert Size (bp)833
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GenBank IDCT010341
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Entrez GeneCryab (a.k.a. Crya-2, Crya2, HspB5, P23)
- Promoter Alpha B-crystallin promoter 800 bp fragment (-833/-1)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer TTCTTTCCTGCGTTATCC
- 3′ sequencing primer CCATTCACATCGCCATTCA
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 98103-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 98103-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mouse aB crystallin 800bp promoter/AcGFP was a gift from Mason Posner (Addgene plasmid # 98103 ; http://n2t.net/addgene:98103 ; RRID:Addgene_98103) -
For your References section:
The zebrafish as a model system for analyzing mammalian and native alpha-crystallin promoter function. Posner M, Murray KL, McDonald MS, Eighinger H, Andrew B, Drossman A, Haley Z, Nussbaum J, David LL, Lampi KJ. PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717/peerj.4093. eCollection 2017. 10.7717/peerj.4093 PubMed 29201567
Map uploaded by the depositor.
