PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||98301||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHMG2(-309,-153)-HIS3-TEFM1<EfmvaS<PGAL1-PGAL10>ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
- Cloning method Ligation Independent Cloning
Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIMVAu1 was a gift from Claudia Vickers (Addgene plasmid # 98301 ; http://n2t.net/addgene:98301 ; RRID:Addgene_98301)
For your References section:Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast. Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE. Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 10.1186/s13068-017-0728-x. eCollection 2017. 728 [pii] PubMed 28239415