PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||98309||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1<ERG8<PGAL10-PGAL1-IDI1-TRPL15A-loxP-ble-loxP-TERG9
SpeciesS. cerevisiae (budding yeast)
- Cloning method Ligation Independent Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please set 1/1/2018 as the initial day available for public access. Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic seq
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIMVAd40R was a gift from Claudia Vickers (Addgene plasmid # 98309 ; http://n2t.net/addgene:98309 ; RRID:Addgene_98309)
For your References section:Engineered protein degradation of farnesyl pyrophosphate synthase is an effective regulatory mechanism to increase monoterpene production in Saccharomyces cerevisiae. Peng B, Nielsen LK, Kampranis SC, Vickers CE. Metab Eng. 2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 10.1016/j.ymben.2018.02.005. 10.1016/j.ymben.2018.02.005 PubMed 29471044