PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||98297||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
- Cloning method Ligation Independent Cloning
Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPMVAu8 was a gift from Claudia Vickers (Addgene plasmid # 98297 ; http://n2t.net/addgene:98297 ; RRID:Addgene_98297)
For your References section:A squalene synthase protein degradation method for improved sesquiterpene production in Saccharomyces cerevisiae. Peng B, Plan MR, Chrysanthopoulos P, Hodson MP, Nielsen LK, Vickers CE. Metab Eng. 2017 Jan;39:209-219. doi: 10.1016/j.ymben.2016.12.003. Epub 2016 Dec 8. 10.1016/j.ymben.2016.12.003 PubMed 27939849