pJT11
(Plasmid
#98308)
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PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 98308 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS425
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRPL3<ScERG20 N127W<PGAL1-PGAL2>ClLIS1>TRPL41B
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Publicly available as of 1/1/2018. Yeast promoters and terminators were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genome.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJT11 was a gift from Claudia Vickers (Addgene plasmid # 98308 ; http://n2t.net/addgene:98308 ; RRID:Addgene_98308) -
For your References section:
Engineered protein degradation of farnesyl pyrophosphate synthase is an effective regulatory mechanism to increase monoterpene production in Saccharomyces cerevisiae. Peng B, Nielsen LK, Kampranis SC, Vickers CE. Metab Eng. 2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 10.1016/j.ymben.2018.02.005. 10.1016/j.ymben.2018.02.005 PubMed 29471044
