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Dnase1L3 R206C pIRES-DsRed
(Plasmid #98847)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98847 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 98847-D50 50 μg of DNA in Tris buffer $495
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DNA 98847-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pIRES2-DsRed2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dnase1L3
  • Alt name
    Dnase gamma
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    914
  • Mutation
    R206C which greatly reduces nuclease activity
  • GenBank ID
    DQ896097.2
  • Entrez Gene
    DNASE1L3 (a.k.a. DHP2, DNAS1L3, LSD, SLEB16)
  • Promoter CMV
  • Tag / Fusion Protein
    • IRES DsRed

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV forward (Genewiz Universal)
  • 3′ sequencing primer IRES- (custom)
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dnase1L3 cloned by PCR initially from Open Biosystems

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dnase1L3 R206C pIRES-DsRed was a gift from Peter Keyel (Addgene plasmid # 98847 ; http://n2t.net/addgene:98847 ; RRID:Addgene_98847)

  • For your References section:

    Dnase1L3 Regulates Inflammasome-Dependent Cytokine Secretion. Shi G, Abbott KN, Wu W, Salter RD, Keyel PA. Front Immunol. 2017 May 8;8:522. doi: 10.3389/fimmu.2017.00522. eCollection 2017. 10.3389/fimmu.2017.00522 PubMed 28533778
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