pQE32-pr-mEosFP (mEosFP-V69T)
(Plasmid #99213)

• Purpose: Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).
• Depositing Lab: Periklis Pantazis
• Publication: Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi: 10.1002/anie.201706121.

Backbone

• Vector backbone: pQE32
• Backbone size w/o insert (bp): 3452
• Total vector size (bp): 4133
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Turbo
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pr-mEosFP
• Alt name: prmEosFP, mEosFP-V69T
• Species: Lobophyllia hemprichii
• Insert Size (bp): 681
• Mutation: monomeric variant with mutation V69T for primed conversion applications
• GenBank ID: AY765217.1
• Promoter: T5

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: AGATTCAATTGTGAGCGG
• 3′ sequencing primer: CCAGATGGAGTTCTGAGG

Resource Information

• Supplemental Documents:
  • pQE32_mEosFP-V69T.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry