Rational Engineering of Photoconvertible Fluorescent Proteins for Dual-Color Fluorescence Nanoscopy Enabled by a Triplet-State Mechanism of Primed Conversion.
Mohr MA, Kobitski AY, Sabater LR, Nienhaus K, Obara CJ, Lippincott-Schwartz J, Nienhaus GU, Pantazis P
Angew Chem Int Ed Engl. 2017 Jun 29. doi: 10.1002/anie.201706121. PubMed Article
Mohr MA, Kobitski AY, Sabater LR, Nienhaus K, Obara CJ, Lippincott-Schwartz J, Nienhaus GU, Pantazis P
Angew Chem Int Ed Engl. 2017 Jun 29. doi: 10.1002/anie.201706121. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99213 | pQE32-pr-mEosFP (mEosFP-V69T) | Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T). |
| 99221 | pRSET-pr-mEos3.1 (mEosFP3.1-A69T) | Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T). |
| 99224 | pRSET-pr-mEos3.2 (mEosFP3.2-A69T) | Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T). |
| 99225 | pRSET-pr-mEos4a (mEosFP4a-V69T) | Bacterial expression of the primed conversion capable protein pr-mEos4a (mEos4a-V69T). |
| 99226 | pRSET-pr-mEos4b (mEosFP4b-V69T) | Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T). |
| 99227 | pRSET-pr-mKikGR1 (mKikGR1-V69T) | Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T). |
| 99228 | pRSET-pr-Kaede (Kaede-A69T) | Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T). |
| 99229 | pCMV-Lifect-7-pr-mEos2 (mEos2-A69T) | Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion. |
| 99230 | Ensconsin-18-mEos2 | Mammalian expression of the photoconvertible protein mEos2 as an Ensconsin fusion. |