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pET-NCBD + ACTR
(Plasmid #99335)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 99335 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET22b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5493
  • Total vector size (bp) 5945
  • Modifications to backbone
    introduced second RBS
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    CBP NCBD (2059-2117)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    183
  • Entrez Gene
    CREBBP (a.k.a. CBP, KAT3A, MKHK1, RSTS, RSTS1)
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    SRC3 (1023-1093)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    219
  • GenBank ID
  • Entrez Gene
    NCOA3 (a.k.a. ACTR, AIB-1, AIB1, CAGH16, CTG26, KAT13B, RAC3, SRC-3, SRC3, TNRC14, TNRC16, TRAM-1, bHLHe42, pCIP)
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-NCBD + ACTR was a gift from Peter Wright (Addgene plasmid # 99335 ; http://n2t.net/addgene:99335 ; RRID:Addgene_99335)
  • For your References section:

    Mutual synergistic folding in recruitment of CBP/p300 by p160 nuclear receptor coactivators. Demarest SJ, Martinez-Yamout M, Chung J, Chen H, Xu W, Dyson HJ, Evans RM, Wright PE. Nature. 2002 Jan 31;415(6871):549-53. 10.1038/415549a PubMed 11823864