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GST-BRD (1079-1198)
(Plasmid #99507)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 99507 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX4T2
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 4970
  • Total vector size (bp) 5336
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mouse CBP BRD (1079-1198)
  • Alt name
    crebbp
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    366
  • Entrez Gene
    Crebbp (a.k.a. AW558298, CBP, CBP/p300, KAT3A, p300/CBP)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGEX
  • 3′ sequencing primer pGEX
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-BRD (1079-1198) was a gift from Peter Wright (Addgene plasmid # 99507 ; http://n2t.net/addgene:99507 ; RRID:Addgene_99507)
  • For your References section:

    The CH2 domain of CBP/p300 is a novel zinc finger. Park S, Martinez-Yamout MA, Dyson HJ, Wright PE. FEBS Lett. 2013 Aug 19;587(16):2506-11. doi: 10.1016/j.febslet.2013.06.051. Epub 2013 Jul 4. 10.1016/j.febslet.2013.06.051 PubMed 23831576