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pET-KIX (586-672)
(Plasmid #99336)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 99336 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Novaen
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 5707
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CBP KIX (586-672)
  • Alt name
    crebbp
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    264
  • Entrez Gene
    Crebbp (a.k.a. AW558298, CBP, CBP/p300, KAT3A, p300/CBP)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-KIX (586-672) was a gift from Peter Wright (Addgene plasmid # 99336 ; http://n2t.net/addgene:99336 ; RRID:Addgene_99336)
  • For your References section:

    Structural analyses of CREB-CBP transcriptional activator-coactivator complexes by NMR spectroscopy: implications for mapping the boundaries of structural domains. Radhakrishnan I, Perez-Alvarado GC, Parker D, Dyson HJ, Montminy MR, Wright PE. J Mol Biol. 1999 Apr 16;287(5):859-65. 10.1006/jmbi.1999.2658 PubMed 10222196