pCGT-HCF-1-C600
(Plasmid #99364)

• Purpose: C-term of HCFC1 protein
• Depositing Lab: Winship Herr
• Publication: Wilson et al Mol Cell Biol. 2000 Sep;20(18):6721-30.

Backbone

• Vector backbone: pCGT
• Backbone size w/o insert (bp): 5068
• Total vector size (bp): 6959
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HCF-1 C-term 1436-2035
• Alt name: HCFC1 C-term
• Alt name: host cell factor C1 C-term
• Species: H. sapiens (human)
• Insert Size (bp): 1891
• Mutation: Deletion of HCFC1 cDNA
• GenBank ID: NM_005334.2 NP_005325.2
• Entrez Gene: HCFC1 (a.k.a. CFF, HCF, HCF-1, HCF1, HFC1, MAHCX, MRX3, PPP1R89, VCAF, XLID3)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 2x tandem T7 tag (N terminal on backbone)
  • C-myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV_for

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry