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pSuper-Retro-Puro-Drp1-shRNA
(Plasmid #99385)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99385 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSuper-Retro-Puro
  • Backbone manufacturer
    Oligoengine
  • Backbone size w/o insert (bp) 6313
  • Total vector size (bp) 6374
  • Vector type
    Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dynamin-1-like protein
  • Alt name
    Drp1
  • gRNA/shRNA sequence
    CAGGAGCCAGCTAGATATTAA
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_012062
  • Entrez Gene
    DNM1L (a.k.a. DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5)
  • Promoter H1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GGAAGCCTTGGCTTTTG
  • 3′ sequencing primer CGAACGCTGACGTCATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The shRNA target sequence employed in this vector was first identified and used in: Friedman, etal. Science 2011, 334, 358–362. (www.ncbi.nlm.nih.gov/pubmed/21885730)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSuper-Retro-Puro-Drp1-shRNA was a gift from David Kashatus (Addgene plasmid # 99385 ; http://n2t.net/addgene:99385 ; RRID:Addgene_99385)

  • For your References section:

    Proteome-wide copy-number estimation from transcriptomics. Sweatt AJ, Griffiths CD, Groves SM, Paudel BB, Wang L, Kashatus DF, Janes KA. Mol Syst Biol. 2024 Sep 27. doi: 10.1038/s44320-024-00064-3. 10.1038/s44320-024-00064-3 PubMed 39333715
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