pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2
PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||99694||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepAAV (pUC f1)
Vector typeAAV, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert namedCas9 and gRNA targeting Actc1
Entrez GeneActc1 (a.k.a. Actc-1)
/ Fusion Protein
- VPR mini (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2 was a gift from George Church (Addgene plasmid # 99694 ; http://n2t.net/addgene:99694 ; RRID:Addgene_99694)