Synchronization of secretory protein traffic in populations of cells.
Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche L, Mercanti V, Jollivet F, Raposo G, Perez F
Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 65252 | Str-KDEL_ManII-SBP-EGFP | synchronize trafficking of ManII from the ER (RUSH system) |
| 65253 | Str-KDEL_ManII-SBP-mCherry | synchronize trafficking of ManII from the ER (RUSH system) |
| 65254 | Str-KDEL_ManII-SBP-tagBFP | synchronize trafficking of ManII from the ER (RUSH system) |
| 65255 | Ii-Str_ManII-SBP-EGFP | synchronize trafficking of ManII from the ER (RUSH system) |
| 65256 | Ii-Str_ManII-SBP-mCherry | synchronize trafficking of ManII from the ER |
| 65257 | Ii-Str_ManII-SBP-tagBFP | synchronize trafficking of ManII from the ER (RUSH system) |
| 65258 | pCDH_Str-KDEL_ManII-SBP-EGFP | synchronize trafficking of ManII from the ER (RUSH system) |
| 65259 | pCDH_Str-KDEL_ManII-SBP-mCherry | synchronize trafficking of ManII from the ER (RUSH system) |
| 65260 | ManII-SBP-EGFP_puromycin | ManII reporter only (no hook) (RUSH system) |
| 65261 | ManII-SBP-mCherry_puromycin | ManII reporter only (no hook) (RUSH system) |
| 65262 | Str-STIM1-NN_ST-SBP-EGFP | synchronize trafficking of ST from the ER (RUSH system) |
| 65263 | Str-STIM1-NN_ST-SBP-mCherry | synchronize trafficking of ST from the ER (RUSH system) |
| 65264 | Str-KDEL_ST-SBP-EGFP | synchronize trafficking of ST from the ER (RUSH system) |
| 65265 | Str-KDEL_ST-SBP-mCherry | synchronize trafficking of ST from the ER (RUSH system) |
| 65266 | Str-KDEL_ST-SBP-tagBFP | synchronize trafficking of ST from the ER (RUSH system) |
| 65267 | pCDH_Str-KDEL_ST-SBP-EGFP | synchronize trafficking of ST from the ER (RUSH system) |
| 65269 | Ii-Str_ST-SBP-mCherry | synchronize trafficking of ST from the ER (RUSH system) |
| 65270 | ST-SBP-EGFP_puromycin | ST reporter only ( no hook) (RUSH system) |
| 65271 | ST-SBP-mCherry_puromycin | ST reporter only ( no hook) (RUSH system) |
| 65272 | Str-KDEL_flGalT-SBP-EGFP | synchronize trafficking of GalT from the ER (RUSH system) |
| 65273 | Str-KDEL_flGalT-SBP-mCherry | synchronize trafficking of GalT from the ER (RUSH system) |
| 65274 | Str-KDEL_flGalT-SBP-tagBFP | synchronize trafficking of GalT from the ER (RUSH system) |
| 65275 | flGalT-SBP-EGFP_puromycin | GalT reporter only ( no hook) (RUSH system) |
| 65276 | flGalT-SBP-mCherry_puromycin | GalT reporter only ( no hook) (RUSH system) |
| 65277 | Ii-Str_ssSBP-EGFP | to synchronize transport of secretory soluble GFP from the ER (RUSH system) |
| 65278 | Str-KDEL_TNF-SBP-EGFP | synchronize trafficking of TNF from the ER (RUSH system) |
| 65279 | Str-KDEL_TNF-SBP-mCherry | synchronize trafficking of TNF from the ER (RUSH system) |
| 65280 | Ii-Str_TNF-SBP-EGFP | synchronize trafficking of TNF from the ER (RUSH system) |
| 65282 | pCDH_TNF-SBP-EGFP | TNF reporter only (no hook) (RUSH system) |
| 65283 | pCDH_TNF-SBP-mCherry | TNF reporter only (no hook) (RUSH system) |
| 65284 | TNF-SBP-EGFP_puromycin | TNF reporter only (no hook) (RUSH system) |
| 65285 | TNF-SBP-mCherry_puromycin | TNF reporter only (no hook) (RUSH system) |
| 65286 | Str-KDEL_SBP-EGFP-Ecadherin | synchronize trafficking of Ecadherin from the ER (RUSH system) |
| 65287 | Str-KDEL_SBP-mCherry-Ecadherin | synchronize trafficking of Ecadherin from the ER (RUSH system) |
| 65288 | Ii-Str_SBP-EGFP-Ecadherin | synchronize trafficking of Ecadherin from the ER (RUSH system) |
| 65289 | Ii-Str_SBP-mCherry-Ecadherin | synchronize trafficking of Ecadherin from the ER (RUSH system) |
| 65290 | pCDH_SBP-EGFP-Ecadherin | Ecadherin reporter only (no hook) (RUSH system) |
| 65291 | pCDH_SBP-mCherry-Ecadherin | Ecadherin reporter only (no hook) (RUSH system) |
| 65292 | SBP-EGFP-Ecadherin_puromycin | Ecadherin reporter only (no hook) (RUSH system) |
| 65293 | SBP-mCherry-Ecadherin_puromycin | Ecadherin reporter only (no hook) (RUSH system) |
| 65294 | Str-KDEL_SBP-EGFP-GPI | synchronize trafficking of EGFP-GPI from the ER (RUSH system) |
| 65295 | Str-KDEL_SBP-mCherry-GPI | synchronize trafficking of mCherry-GPI from the ER (RUSH system) |
| 65296 | Ii-Str_SBP-EGFP-GPI | synchronize trafficking of EGFP-GPI from the ER (RUSH system) |
| 65297 | ii-Str_SBP-mCherry-GPI | synchronize trafficking of mCherry-GPI from the ER (RUSH system) |
| 65298 | pCDH_SBP-EGFP-GPI | synchronize trafficking of EGFP-GPI from the ER (RUSH system) |
| 65299 | pCDH_SBP-mCherry-GPI | synchronize trafficking of mCherry-GPI from the ER (RUSH system) |
| 65300 | Str-Ii_VSVG-SBP-EGFP | synchronize trafficking of VSVG from the ER (RUSH system) |
| 65301 | Str-Ii_VSVG-SBP-mCherry | synchronize trafficking of VSVG from the ER (RUSH system) |
| 65302 | VSVGwt-SBP-EGFP_puromycin | VSVG reporter only (no hook) (RUSH system) |
| 65303 | Str-Ii_SBP-EGFP-Golgin84 | synchronize trafficking of Golgin-84 from the ER (RUSH system) |
| 65304 | Str-Ii_SBP-mCherry-Golgin84 | synchronize trafficking of Golgin-84 from the ER (RUSH system) |
| 65305 | Str-Golgin84_VSVG-SBP-EGFP | synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system) |
| 65306 | Str-KDEL_neomycin | Luminal ER hook only (RUSH system) |
| 65307 | pCDH_Str-KDEL_neomycin | Luminal ER hook only (RUSH system) |
| 65308 | Ii-Str_neomycin | Luminal ER hook only (RUSH system) |
| 65309 | Ii-Str_puromycin | Luminal ER hook only (RUSH system) |
| 65310 | Str-STIM1-NN_puromycin | Luminal ER hook only (RUSH system) |
| 65311 | Str-STIM1-NN_neomycin | Luminal ER hook only (RUSH system) |
| 65312 | Str-Ii_neomycin | Cytoplasmic ER hook only (RUSH system) |
| 65313 | pCDH_Str-Ii | Cytoplasmic ER hook only (RUSH system) |