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Charles Gersbach Lab: Multiplex CRISPR/Cas9 Genome Engineering for Directing Myogenic Cellular Differentiation in Primary Human Cells
Gersbach C
Unpublished

Plasmids from Article

ID Plasmid Purpose  
66631pLV hMyoD-IRES-dsRedCo-expresses human MyoD and dsRed
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66632pLV floxed hMyoD-IRES-dsRedco-expresses CRE excisable human MyoD and dsRed
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66707pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFPexpresses a single gRNA and VP64-dCas9-VP64
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66708pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed
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