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Nuclear localization of gamma-tubulin affects E2F transcriptional activity and S-phase progression.

Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M
FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 2011 Jul 25. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
66883pTER E2F1shRNA humanDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F1
66884pTER E2F2shRNA humanDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F2
66885pTER E2F3shRNA humanDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F3
70666pBS/U6/RNAi gamma-tubulin shRNA mouseReduced the expression of gamma-tubulin in murine cells
70667pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)Human mutant of E2F1 lacking amino acids 193-359
70668pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)Human mutant of E2F1 lacking amino acids 2 to 126
70669pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)Human mutant of E2F1 lacking amino acids 284 to 359
87858pEGFPN1-N-gamma-tubulin (1-333)Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS
87859pEGFPN1-N-gamma-tubulin (334-449)Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS
90313pET21 E2F1Expresses human E2F1 in bacteria

Antibodies from Article