pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)
(Plasmid
#70668)
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PurposeHuman mutant of E2F1 lacking amino acids 2 to 126
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Depositing Lab
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Depositing OrganizationLund University
Located in Sweden -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70668 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 70668-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 70668-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameE2F1 lacking residues 2 to 126
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Alt nameRBP3; E2F-1; RBAP1; RBBP3
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SpeciesH. sapiens (human)
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Insert Size (bp)993
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Mutationlacks amino acids 2-126
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GenBank IDNM_005225
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Entrez GeneE2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)
- Promoter cmv
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII BamHI (not destroyed)
- 3′ cloning site BamHI EcoRI (not destroyed)
- 5′ sequencing primer 5’-TAA TAC GAC TCA CTA TAG GG-3’
- 3′ sequencing primer 5’-TAGAAGGCACAGTCGAGG-3’
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byI did sub clone the E2F1 mutant from the E2F1 gene provided to me by Dr. Joseph R. Nevins
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 70668-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 70668-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI) was a gift from Maria Alvarado-Kristensson (Addgene plasmid # 70668 ; http://n2t.net/addgene:70668 ; RRID:Addgene_70668) -
For your References section:
Nuclear localization of gamma-tubulin affects E2F transcriptional activity and S-phase progression. Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M. FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 2011 Jul 25. 10.1096/fj.11-187484 PubMed 21788450