Nonpolarized signaling reveals two distinct modes of 3D cell migration.
Petrie RJ, Gavara N, Chadwick RS, Yamada KM
J Cell Biol. 2012 Apr 30;197(3):439-55. doi: 10.1083/jcb.201201124.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105290 | Ypet-PBD | To measure Rac or Cdc42 activity by binding and FRET analysis |
| 105291 | Ypet-RBD | To measure Rho activity by binding and FRET analysis |
| 105292 | pECFP-Rac1Q61L | Constitutively active Rac 1 |
| 105293 | pECFP-Rac1Y40C | Control for FRET biosensor analysis - cannot bind downstream effectors |
| 105294 | pECFP-Cdc42Q61L | Constitutively active Cdc42 |
| 105295 | pECFP-Cdc42-Y40C | Control for FRET biosensor analysis - cannot bind downstream effectors |