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Nonpolarized signaling reveals two distinct modes of 3D cell migration.
Petrie RJ, Gavara N, Chadwick RS, Yamada KM
J Cell Biol. 2012 Apr 30;197(3):439-55. doi: 10.1083/jcb.201201124. PubMed Article

Plasmids from Article

ID Plasmid Purpose
105290Ypet-PBDTo measure Rac or Cdc42 activity by binding and FRET analysis
105291Ypet-RBDTo measure Rho activity by binding and FRET analysis
105292pECFP-Rac1Q61LConstitutively active Rac 1
105293pECFP-Rac1Y40CControl for FRET biosensor analysis - cannot bind downstream effectors
105294pECFP-Cdc42Q61LConstitutively active Cdc42
105295pECFP-Cdc42-Y40CControl for FRET biosensor analysis - cannot bind downstream effectors

Antibodies from Article