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A generic protocol for the expression and purification of recombinant RNA in Escherichia coli using a tRNA scaffold.
Ponchon L, Beauvais G, Nonin-Lecomte S, Dardel F
Nat Protoc. 2009;4(6):947-59. doi: 10.1038/nprot.2009.67. Epub 2009 May 28.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
67504pBSKExpress the tRNALys scaffold
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67505pBSMExpress the tRNAMet scaffold
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67506pBSKrnaSephExpress the tRNALys scaffold with the sephadex aptamer tag
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67507pBSMrnaSephExpress the tRNAMet scaffold with the sephadex aptamer tag
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67508pBSMrnaStrpExpress the tRNAMet scaffold with the streptavidin aptamer tag
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