A generic protocol for the expression and purification of recombinant RNA in Escherichia coli using a tRNA scaffold.
Ponchon L, Beauvais G, Nonin-Lecomte S, Dardel F
Nat Protoc. 2009;4(6):947-59. doi: 10.1038/nprot.2009.67. Epub 2009 May 28. PubMed Article
Ponchon L, Beauvais G, Nonin-Lecomte S, Dardel F
Nat Protoc. 2009;4(6):947-59. doi: 10.1038/nprot.2009.67. Epub 2009 May 28. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 67504 | pBSK | Express the tRNALys scaffold |
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| 67505 | pBSM | Express the tRNAMet scaffold |
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| 67506 | pBSKrnaSeph | Express the tRNALys scaffold with the sephadex aptamer tag |
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| 67507 | pBSMrnaSeph | Express the tRNAMet scaffold with the sephadex aptamer tag |
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| 67508 | pBSMrnaStrp | Express the tRNAMet scaffold with the streptavidin aptamer tag |
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