Reconstitution of the mitochondrial calcium uniporter in yeast.
Kovacs-Bogdan E, Sancak Y, Kamer KJ, Plovanich M, Jambhekar A, Huber RJ, Myre MA, Blower MD, Mootha VK
Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8985-90. doi: 10.1073/pnas.1400514111. Epub 2014 Jun 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 67642 | DdMCU | DdMCU-Flag in pACT2 vector human codon optimised |
| 253497 | pRS426-HsEMRE | Expresses HsEMRE in pACT2 vector |
| 253519 | pACT2-DdtS-HsMCU | D.discoideum targeting sequence with C-terminal tagged HsMCU mature protein in pACT2 vector |
| 253538 | pLYS1- DdMCU_AIIA, Cterm Flag | Expresses C-terminal Flag tagged DdMCU_AIIA |
| 253539 | pLJM1 - FLAG(Cterm)- HsMCU, 261A, 264A | Expresses C-terminal FLAG tagged HsMCU with Amino Acid at 261 and 264 changed to A |