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pRS426-HsEMRE
(Plasmid #253497)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253497 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRS426
  • Backbone size w/o insert (bp) 6540
  • Total vector size (bp) 6846
  • Modifications to backbone
    Cloned in a CITI promoter between the the HindIII and EcoRI sites of the original pRS426 backbone
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hs EMRE
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    306
  • GenBank ID
    NP_201575
  • Entrez Gene
    SMDT1 (a.k.a. C22orf32, DDDD, EMRE)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindII (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer GATC CCCGGG ATG GCG TCC GGA GC
  • 3′ sequencing primer GATC GGATCC TTA TCA TTA GTC ATC ATC ATC ATC ATC CTC TGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS426-HsEMRE was a gift from Vamsi Mootha (Addgene plasmid # 253497 ; http://n2t.net/addgene:253497 ; RRID:Addgene_253497)

  • For your References section:

    Reconstitution of the mitochondrial calcium uniporter in yeast. Kovacs-Bogdan E, Sancak Y, Kamer KJ, Plovanich M, Jambhekar A, Huber RJ, Myre MA, Blower MD, Mootha VK. Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8985-90. doi: 10.1073/pnas.1400514111. Epub 2014 Jun 2. 10.1073/pnas.1400514111 PubMed 24889638
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