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Primary ciliogenesis requires the distal appendage component Cep123.
Sillibourne JE, Hurbain I, Grand-Perret T, Goud B, Tran P, Bornens M
Biol Open. 2013 Apr 9;2(6):535-45. doi: 10.1242/bio.20134457. Print 2013 Jun 15.
PubMed

Plasmids from Article

ID Plasmid Purpose  
67787pEGFP-C1-Cep123 (CCDC123/Cep89)This plasmid encodes EGFP fused to the N-terminus of isoform 1 of Cep123 (CCDC123/Cep89)
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69836pMyc-C1-Cep123 (CCDC123/Cep89)This plasmid encodes Cep123 (CCDC123/Cep89) isoform 1 with an N-ter Myc-tag
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73327pMyc-C1-Cep123 (isoform 2)Encodes isoform 2 of Cep123 (Cep89/CCDC123) fused to a myc tag
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73328pMyc-C1-Cep123 (isoform 3)Encodes isoform 3 of Cep123 fused to a myc tag
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73329pEGFP-C2-Cep123-C2 domainEncodes the distal appendage targeting domain of isoform 1 of Cep123 (residues 500-600) fused to EGFP
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Antibodies from Article