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A modular open platform for systematic functional studies under physiological conditions.
Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut P, Herzog F, Leonhardt H, Bultmann S
Nucleic Acids Res. 2015 May 24. pii: gkv550.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
65521attB-GFPPlasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines
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65524attB-­GFP-­Poly(A)Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.
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65525attB-mCh-Poly(A)Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.
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65526attB-­GFP-­Poly(A)‐NeoRNeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.
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65528attB-­mCh-­Poly(A)‐NeoRNeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.
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65529attB‐mCh­‐Poly(A)-­PuroRPuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.
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65531attB-GFP-Dnmt3b1-Poly(A)Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus
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65532attB-GFP-Dnmt3b6-Poly(A)Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus
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65551attB-GFP-Dnmt3b1-Poly(A)-NeoRPlasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection
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65552attB-GFP-Dnmt3b6-Poly(A)-NeoRPlasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection
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65553attB-mCh-Dnmt3b1-Poly(A)-NeoRPlasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection
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65554attB-mCh-Dnmt3b6-Poly(A)-NeoRPlasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection
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65556attB-mCh-Dnmt3b1-Poly(A)-PuroRPlasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selection
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65559attB-GFP-Tet1-Poly(A)Plasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locus
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65560attB-GFP-Tet1d834-1363-Poly(A)Plasmid for Bxb1-mediated recombination of the GFP-Tet1d1054-1363 cDNA into a MIN-tagged locus
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65561attB-GFP-Tet1d834-1053-Poly(A)Plasmid for Bxb1-mediated recombination of the GFP-Tet1d834-1053 cDNA into a MIN-tagged locus
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65624Bxb1-surrogate-reporterFluorescent reporter to monitor the activty of Bxb1
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65625pCAG-tagRFP-NLS-HA-Bxb1Vector for cotranslational expression of tagRFP-Bxb1
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65626pEX-A-U6-gRNAEmpty vector for the expression U6 driven gRNA
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Recombinant Antibodies from Article