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CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae.
Mans R, van Rossum HM, Wijsman M, Backx A, Kuijpers NG, van den Broek M, Daran-Lapujade P, Pronk JT, van Maris AJ, Daran JM
FEMS Yeast Res. 2015 Mar;15(2). pii: fov004. doi: 10.1093/femsyr/fov004. Epub 2015 Mar 4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
107916pMEL10URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107917pMEL11 amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107918pMEL12hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107919pMEL13 kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107920pMEL14 LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107921pMEL15 nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107922pMEL16 HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107923pMEL17 TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107924pROS10 URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107925pROS11 amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107926pROS12 hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107927pROS13 kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107928pROS14 LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107929pROS15 nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107930pROS16 HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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107931pROS17 TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
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Recombinant Antibodies from Article