Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells.
Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R
Genesis. 2006 Jan . 44(1):23-8.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 20730 | Col1a-frt-hygro-pA | |
| 20731 | Col1a-3'probe | |
| 20732 | Col1A-5'probe | |
| 20733 | pCAGGS-flpE-puro | Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker |
| 20734 | pgk-ATG-frt | |
| 21548 | CAGGS-losSTOPlox-ClaI flp-in | |
| 47381 | R26P-M2rtTA | Rosa 26 targeting vector |