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Addgene

R26P-M2rtTA
(Plasmid #47381)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 47381 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    ROSA26-1
  • Backbone size w/o insert (bp) 9846
  • Total vector size (bp) 11946
  • Vector type
    Mouse Targeting
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    M2rtTA
  • Insert Size (bp)
    1500

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    pgk-puro
  • Insert Size (bp)
    1600

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    M2-rtTA was obtained from Dr. H. Bujard
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R26P-M2rtTA was a gift from Rudolf Jaenisch (Addgene plasmid # 47381 ; http://n2t.net/addgene:47381 ; RRID:Addgene_47381)
  • For your References section:

    Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R. Genesis. 2006 Jan . 44(1):23-8. 10.1002/gene.20180 PubMed 16400644