Skip to main content
Addgene
Open Menu

mCol.4F2A targeting construct
(Plasmid #25794)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25794 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 25794-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 25794-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    mCol.loxneo
  • Backbone size w/o insert (bp) 10600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5000

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer tagagaaaagtgaaagtcgagtttac
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCol.4F2A targeting construct was a gift from Rudolf Jaenisch (Addgene plasmid # 25794 ; http://n2t.net/addgene:25794 ; RRID:Addgene_25794)

  • For your References section:

    Single-gene transgenic mouse strains for reprogramming adult somatic cells. Carey BW, Markoulaki S, Beard C, Hanna J, Jaenisch R. Nat Methods. 2010 Jan . 7(1):56-9. 10.1038/nmeth.1410 PubMed 20010831
Related items:
Commonly requested with: