Addgene: Dynamic single cell measurements of kinase activity by synthetic kinase activity relocation sensors.

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Serge Pelet
Durandau et al

Dynamic single cell measurements of kinase activity by synthetic kinase activity relocation sensors.
Durandau E, Aymoz D, Pelet S
BMC Biol. 2015 Aug 1;13:55. doi: 10.1186/s12915-015-0163-z. PubMed

Plasmids from Article

ID	Plasmid	Purpose
68274	pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry	SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry	Loading...
68275	pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry	SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS	Loading...
68276	pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry	SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLS	Loading...
68277	pED55 pRS306 pRPS2-SteND-NLS-mCherry	SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation	Loading...
68278	pED73 pRS304 pRPS2-SteDS-NLS-mCitrine	SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrine	Loading...
68279	pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry	SKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherry	Loading...
68280	pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry	SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry	Loading...

Recombinant Antibodies from Article