Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Dynamic single cell measurements of kinase activity by synthetic kinase activity relocation sensors.
Durandau E, Aymoz D, Pelet S
BMC Biol. 2015 Aug 1;13:55. doi: 10.1186/s12915-015-0163-z. PubMed

Plasmids from Article

ID Plasmid Purpose
68274pED45: pRS306 pRPS2-Ste7DS-NLS-mCherrySKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry
68275pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherrySKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS
68276pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherrySKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLS
68277pED55 pRS306 pRPS2-SteND-NLS-mCherrySKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation
68278pED73 pRS304 pRPS2-SteDS-NLS-mCitrineSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrine
68279pSP303 pRS306 pRPS2-Far1DS-NLS-mCherrySKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherry
68280pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherrySKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

Antibodies from Article