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pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry
(Plasmid #68276)


Item Catalog # Description Quantity Price (USD)
Plasmid 68276 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Ste7DS NLS(4E) mCherry
  • Species
  • Promoter RPS2
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry was a gift from Serge Pelet (Addgene plasmid # 68276 ; ; RRID:Addgene_68276)
  • For your References section:

    Dynamic single cell measurements of kinase activity by synthetic kinase activity relocation sensors. Durandau E, Aymoz D, Pelet S. BMC Biol. 2015 Aug 1;13:55. doi: 10.1186/s12915-015-0163-z. 10.1186/s12915-015-0163-z [pii] PubMed 26231587