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Conditional protein splicing of alpha-sarcin in live cells.
Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 2014 Jan 30. PubMed Article

Plasmids from Article

ID Plasmid Purpose
70223pcDNA3-FRB-VMAc-CSarcinExpresses VMA intein with C-terminal portion of alpha-sarcin
70225pcDNA3-NGFP-VMAn-FKBPExpresses N-terminal portion of GFP along with VMA intein for protein splicing
70226pcDNA3-FRB-VMAc-CGFPExpresses C-terminal portion of GFP along with VMA intein for protein splicing
702293XFLAG-NSarQ27G-VMAn-FKBPExpresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
70230NSarQ27G-VMAn-FKBPExpresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

Antibodies from Article