pcDNA3-FRB-VMAc-CGFP
(Plasmid
#70226)
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PurposeExpresses C-terminal portion of GFP along with VMA intein for protein splicing
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Depositing Lab
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Depositing OrganizationUniversity of Victoria
Located in Canada -
Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 70226-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 70226-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6253
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeGFP
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SpeciesSynthetic
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Insert Size (bp)333
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MutationChanged Ile 129 to Cys, Deleted amino acids 1-128
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GenBank IDLC008492.1
- Promoter CMV
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Tags
/ Fusion Proteins
- FKBP-rapamycin binding (FRB) domain of the mammalian target of rapamycin (mTOR) kinase (N terminal on insert)
- Linker Peptide (N terminal on insert)
- VMAc (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer C-Sar For
- 3′ sequencing primer C-Sar Rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 70226-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 70226-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-FRB-VMAc-CGFP was a gift from Perry Howard (Addgene plasmid # 70226 ; http://n2t.net/addgene:70226 ; RRID:Addgene_70226) -
For your References section:
Conditional protein splicing of alpha-sarcin in live cells. Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL. Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 2014 Jan 30. 10.1039/c3mb70387h PubMed 24481070