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Bacterial RNA polymerase can retain sigma70 throughout transcription.
Harden TT, Wells CD, Friedman LJ, Landick R, Hochschild A, Kondev J, Gelles J
Proc Natl Acad Sci U S A. 2016 Jan 5. pii: 201513899. PubMed Article

Plasmids from Article

ID Plasmid Purpose  
70061pCDW114artificial transcription template "wild-type"
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70062pCDW115artificial transcription template "pause ablation"
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70063pTH07artificial transcription template "distal pause"
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70064pTH09artificial transcription template "terminator insertion"
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