Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pTH07
(Plasmid #70063)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 70063 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFW11 Tet

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Tetracycline, 25 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lambda PR' - repeat cassette - E. coli rpoB with inserted pause - lambda TR'
  • Species
    Synthetic
  • GenBank ID
    KT326915

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH07 was a gift from Jeff Gelles (Addgene plasmid # 70063 ; http://n2t.net/addgene:70063 ; RRID:Addgene_70063)
  • For your References section:

    Bacterial RNA polymerase can retain sigma70 throughout transcription. Harden TT, Wells CD, Friedman LJ, Landick R, Hochschild A, Kondev J, Gelles J. Proc Natl Acad Sci U S A. 2016 Jan 5. pii: 201513899. 10.1073/pnas.1513899113 PubMed 26733675