Fluorogen activating protein-affibody probes: modular, no-wash measurement of epidermal growth factor receptors.
Wang Y, Telmer CA, Schmidt BF, Franke JD, Ort S, Arndt-Jovin DJ, Bruchez MP
Bioconjug Chem. 2015 Jan 21;26(1):137-44. doi: 10.1021/bc500525b. Epub 2014 Dec 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 73214 | pET21-10XHis-GST-HRV-dL5 | Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP) |
| 73215 | pET21-10XHis-GST-HRV-dL5-Her1 | Bacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP) |
| 73216 | pET21-10XHis-GST-HRV-Her1-dL5 | Bacterial expression vector for Z1907-dL5(E52D) affibody fusion protein (AF) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP) |
| 73217 | pET21-10XHis-GST-HRV-Her1-dL5-Her1 | Bacterial expression vector for Z1907-dL5(E52D)-Z1907 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP) |
| 73218 | pET21-10XHis-GST-HRV-dHer1cys | Bacterial expression vector for Z1907 affibody protein (A) with N-terminal His10, GST and HRV3C cleavage site (cysteine tag) |