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Multiple Gene Repression in Cyanobacteria Using CRISPRi.
Yao L, Cengic I, Anfelt J, Hudson EP
ACS Synth Biol. 2015 Dec 28.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
73220pMD19T-psba1-Ppsba2-dCas9-SpRContains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
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73221pMD19T-slr0230-PL31-sgRNANT1-KmRContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
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73222pMD19T-slr0168-Ptrc-GFP3mut-CmRContains GFPmut3b under the Ptrc promoter. Suicide vector inserts into slr0168 site of Synechocystis. Carries chloramphenicol resistance. Propogates in E. coli
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73223pMD19T-psba1-TetR-PL22-dCas9-SpRContains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
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73224pMD19T-slr0230-PL22-sgRNANT1-KmRContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
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