Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Multiple Gene Repression in Cyanobacteria Using CRISPRi.
Yao L, Cengic I, Anfelt J, Hudson EP
ACS Synth Biol. 2015 Dec 28.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
73220pMD19T-psba1-Ppsba2-dCas9-SpRContains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
Loading...
73221pMD19T-slr0230-PL31-sgRNANT1-KmRContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
Loading...
73222pMD19T-slr0168-Ptrc-GFP3mut-CmRContains GFPmut3b under the Ptrc promoter. Suicide vector inserts into slr0168 site of Synechocystis. Carries chloramphenicol resistance. Propogates in E. coli
Loading...
73223pMD19T-psba1-TetR-PL22-dCas9-SpRContains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
Loading...
73224pMD19T-slr0230-PL22-sgRNANT1-KmRContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
Loading...

Antibodies from Article