Multiple Gene Repression in Cyanobacteria Using CRISPRi.
Yao L, Cengic I, Anfelt J, Hudson EP
ACS Synth Biol. 2015 Dec 28. PubMed Article
Yao L, Cengic I, Anfelt J, Hudson EP
ACS Synth Biol. 2015 Dec 28. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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73220 | pMD19T-psba1-Ppsba2-dCas9-SpR | Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation. |
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73221 | pMD19T-slr0230-PL31-sgRNANT1-KmR | Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli |
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73222 | pMD19T-slr0168-Ptrc-GFP3mut-CmR | Contains GFPmut3b under the Ptrc promoter. Suicide vector inserts into slr0168 site of Synechocystis. Carries chloramphenicol resistance. Propogates in E. coli |
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73223 | pMD19T-psba1-TetR-PL22-dCas9-SpR | Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation. |
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73224 | pMD19T-slr0230-PL22-sgRNANT1-KmR | Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli |
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