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Consequences of Cas9 cleavage in the chromosome of Escherichia coli.
Cui L, Bikard D
Nucleic Acids Res. 2016 Apr 8. pii: gkw223.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
78492pCRRNAcosTranscription of Streptococcus pyogenes crRNA and tracrRNA under the wildtype crRNA promoter.
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78493pZA31-sulA-GFPExpression of GFP under the sulA promoter.
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78494pLC13Expression of Mu-gam under the pBAD promoter
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78551LC-E02E. coli strain K-12 MG1655, pTet--dCas9 cassette integrated at 186 primary attB site.
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78552LC-E03E. coli strain K-12 MG1655, pTet--Cas9 cassette integrated at 186 primary attB site.
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Antibodies from Article