pLC13
(Plasmid
#78494)
-
PurposeExpression of Mu-gam under the pBAD promoter
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78494 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepBAD18
- Total vector size (bp) 5120
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMu-gam
-
Entrez GeneMup10 (a.k.a. Mup10)
Resource Information
-
A portion of this plasmid was derived from a plasmid made bypBAD18 (Guzman, 1995, J. Bacteriol)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Mu-gam insert within this plasmid contains an L126R mutation. The depositor states that this mutation does not affect plasmid function.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLC13 was a gift from David Bikard (Addgene plasmid # 78494 ; http://n2t.net/addgene:78494 ; RRID:Addgene_78494) -
For your References section:
Consequences of Cas9 cleavage in the chromosome of Escherichia coli. Cui L, Bikard D. Nucleic Acids Res. 2016 Apr 8. pii: gkw223. 10.1093/nar/gkw223 PubMed 27060147
