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Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch.
Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, Savage DF
Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
74490pBLO1811_Cas9_noNLS_humanHuman codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA
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74491pBLO1808_arC9_2xNLS_humanHuman codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA
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74493pBLO1806_Cas9_2xNLS_humanHuman codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA
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74494pBLO1811_arC9_noNLS_humanHuman codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA
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117170pBC2101-reciU6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLS
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117171pBC2102-reciU6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS
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117172pBC2103-reciU6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS
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121418pBLO5.01pUC19_BsaI-dCas9-BsaI
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121495pBLO 4.01engineered Mu transposon
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121496pBLO 9.2_bCas9-CP199TetR_tetR/tetA promoters_dCas9-CP_CM_P15A
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121497pBLO 9.3_bCas9-CP230TetR_tetR/tetA promoters_dCas9-CP_CM_P15A
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121498pBLO 9.6_bCas9-CP1010TetR_tetR/tetA promoters_dCas9-CP_CM_P15A
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121500pBLO 9.15_bCas9-CP1249TetR_tetR/tetA promoters_dCas9-CP_CM_P15A
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121501pBLO 9.16_bCas9-CP1282TetR_tetR/tetA promoters_dCas9-CP_CM_P15A
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121624pBLO 36.02_ProCas9TEVTetR_tetR/tetA promoters_bCas9-CP199-TEVlinker-2xflag_CM_P15A
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121629pBLO43.3_human ProCas9FlaviU6-sgRNAdest_CMV-intron_hProCas9Flavi_T2A Mcherry_AmpR_ColE1
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121630pBLO43.3.-6_human ProCas9Flavi-6U6-sgRNAdest_CMV-intron_hProCas9Flavi-6_T2A Mcherry_AmpR_ColE1
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121631pBLO44.1_dProteaseCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1
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121632pBLO44.3_TuMVproCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1
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121633pBLO44.4_PPVproCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1
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121634pBLO44.5_PVYproCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1
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121635pBLO44.6_ZIKVproCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1
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121638pBLO44.7_WNVproCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1
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Antibodies from Article