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A simple DNA handle attachment method for single molecule mechanical manipulation experiments.
Min D, Arbing MA, Jefferson RE, Bowie JU
Protein Sci. 2016 Aug;25(8):1535-44. doi: 10.1002/pro.2952. Epub 2016 Jun 6. PubMed Article

Plasmids from Article

ID Plasmid Purpose
79053pMBP-SpyCatcherExpresses maltose binding protein (MBP) SpyCatcher fusion
79054pSpyTag-GlpGExpresses SpyTagged GlpG protein

Antibodies from Article