pSpyTag-GlpG
(Plasmid #79054)

• Purpose: Expresses SpyTagged GlpG protein
• Depositing Lab: James Bowie
• Publication: Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi: 10.1002/pro.2952. Epub 2016 Jun 6.

Backbone

• Vector backbone: pTrcHisB
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4269
• Total vector size (bp): 4992
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SpyTag-GlpG
• Insert Size (bp): 723
• Mutation: GlpG protein employed here corresponds to residues 87-276 of E. coli GlpG.
• Entrez Gene: glpG (a.k.a. b3424, ECK3410)
• Promoter: Trc
• Tags / Fusion Proteins:
  • 6xHis (N terminal on insert)
  • SpyTag (N terminal on insert)
  • SpyTag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13pUC-Rev
• 3′ sequencing primer: pBAD-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Transform pSpyTag-GlpG plasmid into E. coli BL21-Gold (DE3) for expression.