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Binding of STIL to Plk4 activates kinase activity to promote centriole assembly.
Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
80264pFastBac GST-Plk4baculovirus creation plasmid for GST-Plk4
80265pFastBac GST-STILbaculovirus creation plasmid for GST-STIL
80266pcDNA5 FRT/TO MycLAP-STILmammalian expression plasmid for MycLAP-STIL
80267pcDNA5 FRT/TO MycLAP-STIL 5Amammalian expression plasmid for MycLAP-STIL 5A
80268pcDNA5 FRT/TO MycLAP-STIL ∆CCmammalian expression plasmid for MycLAP-STIL coiled-coil deletion
80269pcDNA5 FRT/TO Plk4-mCherrymammalian expression plasmid for Plk4-mCherry
80270pcDNA5 FRT/TO Plk4 KD-mCherrymammalian expression plasmid for kinase dead Plk4
80271pcDNA5 FRT/TO Plk4 ∆24-mCherrymammalian expression plasmid for Plk4 with multi-phospho degron deleted
80272pcDNA5 FRT/TO Plk4 KD ∆24-mCherrymammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted
80273pFastBac GST-SAS6baculovirus creation plasmid for GST-SAS6

Antibodies from Article