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pcDNA5 FRT/TO MycLAP-STIL 5A
(Plasmid #80267)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80267 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5 FRT/TO
  • Backbone size w/o insert (bp) 6126
  • Total vector size (bp) 9975
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STIL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3861
  • Mutation
    S1103A, S1108A, S1111A, S1116A, T1119A
  • Entrez Gene
    STIL (a.k.a. MCPH7, SIL)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc-GFP (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5 FRT/TO MycLAP-STIL 5A was a gift from Andrew Holland (Addgene plasmid # 80267 ; http://n2t.net/addgene:80267 ; RRID:Addgene_80267)
  • For your References section:

    Binding of STIL to Plk4 activates kinase activity to promote centriole assembly. Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ. J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088. 10.1083/jcb.201502088 PubMed 26101219