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Engineered cellular gene-replacement platform for selective and inducible proteolytic profiling.
Morgan CW, Diaz JE, Zeitlin SG, Gray DC, Wells JA
Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8344-9. doi: 10.1073/pnas.1504141112. Epub 2015 Jun 23. PubMed Article

Plasmids from Article

ID Plasmid Purpose
100095Frb-N-TEV SNIPer plasmid, N terminal TEV fused to Frb
100096FKBP-C-TEV 219 SNIPer plasmid, C terminal TEV fused to FKBP
100098pETDuet_huCAD_ICADL (TevS)dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

Antibodies from Article