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pETDuet_huCAD_ICADL (TevS)
(Plasmid #100098)


Item Catalog # Description Quantity Price (USD)
Plasmid 100098 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    DNA fragmentation factor subunit alpha
  • Species
    H. sapiens (human)
  • Mutation
    two caspase cleavage sites (DETD-117 and DAVD-224) in ICAD were sequentially mutated to TEVP cleavable sequences
  • Entrez Gene
    CAD (a.k.a. CDG1Z, DEE50, EIEE50, GATD4)
  • Entrez Gene
    DFFA (a.k.a. DFF-45, DFF1, ICAD)
  • Promoter T7

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note- insertion of amino acids ENLYFG after AA117, and insertion of ENLYFQSG after AA224 in ICAD [Genbank sequence NP_004392.1.] are expected insertions. They make the ICAD variant recognizable and cleavable by TEVP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet_huCAD_ICADL (TevS) was a gift from Jim Wells (Addgene plasmid # 100098 ; ; RRID:Addgene_100098)
  • For your References section:

    Engineered cellular gene-replacement platform for selective and inducible proteolytic profiling. Morgan CW, Diaz JE, Zeitlin SG, Gray DC, Wells JA. Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8344-9. doi: 10.1073/pnas.1504141112. Epub 2015 Jun 23. 10.1073/pnas.1504141112 PubMed 26106156